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10 μm sb-505124  (PeproTech)


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    Structured Review

    PeproTech 10 μm sb-505124
    Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB <t>SB-505124</t> hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™
    10 μm Sb 505124, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10+%CE%BCm+sb-505124/pmc05747074-63-19-29?v=PeproTech
    Average 90 stars, based on 1 article reviews
    10 μm sb-505124 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method"

    Article Title: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-017-0738-4

    Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB SB-505124 hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™
    Figure Legend Snippet: Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB SB-505124 hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™

    Techniques Used: In Vivo, In Vitro, Recombinant, Knock-Out

    Directed differentiation of feeder-free hPSCs led to rapid hPSC-LESC production. a Schematic illustration of the differentiation strategy. b Phase-contrast images showing cell morphology after 22 days of differentiation for hESC1-LESCs; scale bars = 100 μm. c Expression of LESC markers after 22 days of differentiation. Nuclei counterstained with DAPI; scale bars = 100 μm. d Percentage of p40- and p63α-positive cells quantified from cytospin samples after 24 days of differentiation; n = 10 images, 1986 cells. bFGF basic fibroblast growth factor, Blebb. blebbistatin, BMP bone morphogenetic protein, IF immunofluorescence, SB SB-505124 hydrochloride hydrate
    Figure Legend Snippet: Directed differentiation of feeder-free hPSCs led to rapid hPSC-LESC production. a Schematic illustration of the differentiation strategy. b Phase-contrast images showing cell morphology after 22 days of differentiation for hESC1-LESCs; scale bars = 100 μm. c Expression of LESC markers after 22 days of differentiation. Nuclei counterstained with DAPI; scale bars = 100 μm. d Percentage of p40- and p63α-positive cells quantified from cytospin samples after 24 days of differentiation; n = 10 images, 1986 cells. bFGF basic fibroblast growth factor, Blebb. blebbistatin, BMP bone morphogenetic protein, IF immunofluorescence, SB SB-505124 hydrochloride hydrate

    Techniques Used: Expressing, Immunofluorescence



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    10 μm sb-505124  (PeproTech)
    90
    PeproTech 10 μm sb-505124
    Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB <t>SB-505124</t> hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™
    10 μm Sb 505124, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10+%CE%BCm+sb-505124/pmc05747074-63-19-29?v=PeproTech
    Average 90 stars, based on 1 article reviews
    10 μm sb-505124 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB SB-505124 hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™

    Journal: Stem Cell Research & Therapy

    Article Title: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

    doi: 10.1186/s13287-017-0738-4

    Figure Lengend Snippet: Schematic illustration of in vivo development and in vitro differentiation of RPE and LESCs. a At early stages of embryonic eye development, surface ectoderm thickens and invaginates together with the underlying neuroepithelium of the optic vesicle. The bilayered optic cup gives rise to the neural retina and the retinal pigment epithelium ( RPE ), while the lens and corneal epithelium develop from the surface ectoderm . Some of the known signaling pathways affecting the cell fate choice during differentiation, such as bone morphogenetic protein ( BMP ) for surface ectoderm, are shown. b An overview of the optimized human pluripotent stem cell ( hPSC ) culture and directed RPE and limbal epithelial stem cell ( LESC ) differentiation protocols, as well as key media and matrix components used (not to scale). Blebb. blebbistatin, col IV collagen type IV, E8 Essential 8™ Flex Medium, FGF fibroblast growth factor, LN-521 recombinant laminin-521, SB SB-505124 hydrochloride hydrate, TGFβ transforming growth factor beta, XF-Ko-SR Knock-out™ serum replacement XenoFree CTS™

    Article Snippet: For LESC differentiation, EBs were subjected to surface ectodermal induction: 1 day in XF-Ko-SR medium supplemented with 10 μM SB-505124 and 50 ng/ml human basic fibroblast growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ, USA), followed by 2 days in XF-Ko-SR medium supplemented with 25 ng/ml bone morphogenetic protein (BMP)-4 (PeproTech Inc.).

    Techniques: In Vivo, In Vitro, Recombinant, Knock-Out

    Directed differentiation of feeder-free hPSCs led to rapid hPSC-LESC production. a Schematic illustration of the differentiation strategy. b Phase-contrast images showing cell morphology after 22 days of differentiation for hESC1-LESCs; scale bars = 100 μm. c Expression of LESC markers after 22 days of differentiation. Nuclei counterstained with DAPI; scale bars = 100 μm. d Percentage of p40- and p63α-positive cells quantified from cytospin samples after 24 days of differentiation; n = 10 images, 1986 cells. bFGF basic fibroblast growth factor, Blebb. blebbistatin, BMP bone morphogenetic protein, IF immunofluorescence, SB SB-505124 hydrochloride hydrate

    Journal: Stem Cell Research & Therapy

    Article Title: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

    doi: 10.1186/s13287-017-0738-4

    Figure Lengend Snippet: Directed differentiation of feeder-free hPSCs led to rapid hPSC-LESC production. a Schematic illustration of the differentiation strategy. b Phase-contrast images showing cell morphology after 22 days of differentiation for hESC1-LESCs; scale bars = 100 μm. c Expression of LESC markers after 22 days of differentiation. Nuclei counterstained with DAPI; scale bars = 100 μm. d Percentage of p40- and p63α-positive cells quantified from cytospin samples after 24 days of differentiation; n = 10 images, 1986 cells. bFGF basic fibroblast growth factor, Blebb. blebbistatin, BMP bone morphogenetic protein, IF immunofluorescence, SB SB-505124 hydrochloride hydrate

    Article Snippet: For LESC differentiation, EBs were subjected to surface ectodermal induction: 1 day in XF-Ko-SR medium supplemented with 10 μM SB-505124 and 50 ng/ml human basic fibroblast growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ, USA), followed by 2 days in XF-Ko-SR medium supplemented with 25 ng/ml bone morphogenetic protein (BMP)-4 (PeproTech Inc.).

    Techniques: Expressing, Immunofluorescence